QPix 420 Bench-top Colony Picker from Molecular Devices LLC

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ItemQPix 420 Bench-top Colony Picker
CompanyMolecular Devices LLC
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Catalog NumberQPix 420

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Molecular Devices LLC

3860 N First Street

San Jose, California 95134

United States

Phone: 408-747-1700

Company Profile

Website: http://www.moleculardevices.com/

(1) Continuous synthesis of E. coli genome sections and Mb-scale human DNA assemblyNatureJuly 20, 2023Jérôme F. Zürcher, Askar A. Kleefeldt, Louise F. H. Funke, Jakob Birnbaum, Julius Fredens, Simona Grazioli, Kim C. Liu, Martin Spinck, Gianluca Petris, Pierre Murat, et al.subsection of the plate and linearly scaling to the whole surface or using automated counting on the QPIX420 system (Molecular Devices; settings: debris, ~0.2; axis ratio, ~0.25). For experiments in recA-deletion

(2) Antibody escape and cryptic cross-domain stabilization in the SARS-CoV-2 Omicron spike proteinCell Host & MicrobeSeptember 14, 2022Kamyab Javanmardi et al.525 (Beckman Coulter) acoustic liquid handler, a Tecan Fluent robotic liquid handler (Tecan), and the QPix 420 Colony Picker (Molecular Devices) to assemble Golden Gate constructs. We arranged parts, either IDT

(3) Visualizing the pH in Escherichia coli Colonies via the Sensor Protein mCherryEA Allows High-Throughput Screening of Mutant LibrariesmSystemsJune 28, 2022Fabian Stefan Franz Hartmann, Tamara Weiß, Jing Shen, Dóra Smahajcsik, Simonas Savickas, Gerd Michael Seiboldmedium. Cells from single colonies on transformation plates were picked up using a colony-picking robot (QPix 420; Molecular Devices. LLC, USA) mounted with a bacterial 96-pin picking head and then transferred to the

(4) Synthetic repertoires derived from convalescent COVID-19 patients enable discovery of SARS-CoV-2 neutralizing antibodies and a novel quaternary binding modalitybioRxivMay 18, 2022Jule Goike et al.on SD -Leu and 8–32 colonies per plate were picked into 2 mL microplates either by hand or using a QPIX 420 (Molecular Devices) automatic colony picker. Cultures were grown at 1000 rpm at 3 mm orbit at 30°C overnight.

(5) A cell-based phenotypic library selection and screening approach for the de novo discovery of novel functional chimeric antigen receptorsScientific reportsJanuary 21, 2022Fierle JK, Abram-Saliba J, Atsaves V, Brioschi M, de Tiani M, Reichenbach P, Irving M, Coukos G, Dunn SM.containing phagemid vectors expressing scFvs under control of a Lac promotor were picked by robot (Qpix420, Molecular Devices) into 96-well PP U-form microplates (Greiner, cat. 650,201) containing 200 µL of

(6) Rapid discovery of diverse neutralizing SARS-CoV-2 antibodies from large-scale synthetic phage librariesmAbsJanuary 1, 2022Yuan TZ, Garg P, Wang L, Willis JR, Kwan E, Hernandez AGL, Tuscano E, Sever EN, Keane E, Soto C, et al.were isolated on 2YT agar plates with 100 µg/ml carbenicillin and single colonies were picked using QPix 420 (Molecular Devices) into 384-well plates containing 2YT with M13KO7 helper phage (Antibody Design Labs)

(7) Rapid characterization of spike variants via mammalian cell surface displayMolecular CellDecember 16, 2021Kamyab Javanmardi et al.incubator for growth overnight (12–16 h). Colonies were screened the following day and picked using the QPix 420 (Molecular Devices), selecting only white colonies and avoiding green fluorescent colonies, which still

(8) Engineering the protein dynamics of an ancestral luciferaseNature CommunicationsDecember 1, 2021Andrea Schenkmayerova et al.the manual screening described above using a colony picking robot (Colony Picker, Molecular Devices QPix 420), a liquid handling robot (Bravo, Agilent) with a 96-tip head and the LARA robotic system (Greifswald,

(9) A Common Pathway for Activation of Host-Targeting and Bacteria-Targeting Toxins in Human Intestinal BacteriamBioSeptember 1, 2021Yiqiao Bao, Andrew A. Verdegaal, Brent W. Anderson, Natasha A. Barry, Jing He, Xiang Gao, Andrew L. Goodmanplates (termed BfN::TN plates) containing TYG medium with erythromycin using a microbial colony picker (QPix 420; Molecular Devices, San Jose, CA, USA). After 20 h anaerobic incubation at 37°C, an aliquot from each

(10) High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid LibraryG3 Genes|Genomes|GeneticsDecember 1, 2020Joseph M. Varberg, Jennifer M. Gardner, Scott McCroskey, Snehabala Saravanan, William D. Bradford, Sue L. Jaspersenplated onto 48-well Bioassay Qtrays (Molecular Devices). Automated colony picking was performed using a QPix 420 robotic colony picker (Molecular Devices), followed by high-throughput plasmid prep using a BioMek FXP

(11) Isobutanol production freed from biological limits using synthetic biochemistryNature CommunicationsDecember 1, 2020Saken Sherkhanov, Tyler P. Korman, Sum Chan, Salem Faham, Hongjiang Liu, Michael R. Sawaya, Wan-Ting Hsu, Ellee Vikram, Tiffany Cheng, James U. Bowieadditions. Colonies for screening of mutant variants were selected automatically using a Molecular Devices Qpix 420 colony picker. Cloning and purification of enzymes The enzymes used in this study are listed in Table

(12) Secretory Expression Fine-Tuning and Directed Evolution of Diacetylchitobiose Deacetylase by Bacillus subtilisApplied and Environmental MicrobiologySeptember 1, 2019Zhu Jiang, Tengfei Niu, Xueqin Lv, Yanfeng Liu, Jianghua Li, Wei Lu, Guocheng Du, Jian Chen, Long Liuusing derivatization reagent ortho-phthalaldehyde (OPA). The screening process was performed by the QPix 420 system (Molecular Devices, Sunnyvale, CA). Single colonies from the mutant library obtained in the above-described

(13) Mapping human microbiome drug metabolism by gut bacteria and their genesNatureJune 3, 2019Michael Zimmermann, Maria Zimmermann-Kogadeeva, Rebekka Wegmann, Andrew L. Goodmanformat into liquid LB medium supplemented with kanamycin using a colony picking robot (Molecular Devices QPix 420). After incubation overnight at 37°C, plates were replicated in duplicate onto LB agar plates supplemented

(14) Alteration of Chain Length Selectivity ofCandida antarcticaLipase A by Semi‐Rational Design for the Enrichment of Erucic and Gondoic Fatty AcidsAdvanced Synthesis & CatalysisNovember 5, 2018Katja Zorn, Isabel Oroz‐Guinea, Henrike Brundiek, Mark Dörr, Uwe T. Bornscheuerinoculate master MTP (M‐MTP) containing 220 μl per well of LB with ampicillin (0.1 mg/ml). Utilizing the QPix 420 Colony Picking System (Molecular Devices, USA) each well was inoculated with only one colony. The so

(15) Rational identification of aggregation hotspots based on secondary structure and amino acid hydrophobicityScientific ReportsDecember 1, 2017Daisuke Matsui, Shogo Nakano, Mohammad Dadashipour, Yasuhisa Asanoon LB agar plates containing 100 μg/ml ampicillin. Colonies were picked from the agar plates using a QPix 420 Bench-top Colony Picker (Molecular Devices, CA, USA) and placed into 96-well plates with 300 μl of LB medium containing 50 μg/ml

(16) Fast, Continuous, and High-Throughput (Bio)Chemical Activity Assay for N -Acyl- l -Homoserine Lactone Quorum-Quenching EnzymesApplied and Environmental MicrobiologyJuly 15, 2016Daniel Last, Georg H. E. Krüger, Mark Dörr, Uwe T. Bornscheuerwas estimated to be about 1:125. Clones were transferred with a 96-pin head microbial colony picker QPix 420 (Molecular Devices, Sunnyvale, CA, USA) from agar plates into the wells of microtiter plates (MTPs).

(17) Alteration of the Donor/Acceptor Spectrum of the (S)-Amine Transaminase from Vibrio fluvialisInternational Journal of Molecular SciencesNovember 11, 2015Maika Genz, Clare Vickers, Tom van den Bergh, Henk-Jan Joosten, Mark Dörr, Matthias Höhne, Uwe T. Bornscheuerincubator and incubated until the colonies had the appropriate size for the use of the colony picker (QPix 420, Molecular Devices, Sunnyvale, CA, USA). Sterile 96-well microtiter plates were filled with 200 µL/well

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